Labeled & Conjugated antibody

OEM Reagents

Antibody Affinity

T3 antibodies R513c8, R514n5, and R515n5 were detected via ELISA platform using the T3 antigen with EC50s of 6.86, 7.71, and 50.8 ng/mL, respectively.

Direct Chemiluminescent Immunoassay (CLIA) platform

Roche-assigned fT3/tT3 clinical samples were detected using the R515n5 (Capture)-R514n5 (Detection) antibody pair. The T3 Sandwich antibodies (R515n5, R514n5) on the direct Chemiluminescent Immunoassay (CLIA) platform demonstrated a 10 times higher signal-to-noise ratio (S/N) compared to the Competitive Immunoassay. The fT3 detection sensitivity achieved is 0.1 pmol/L, and a strong correlation (R² > 0.99) was confirmed between the detection results and Roche-assigned samples.

fT3 Lowest Detection Limit

fT3 Sample Coincidence Rate

tT3 Sample Coincidence Rate

Cross Reactivity

L-T4 at 500ng/mL was measured via T4 sandwich antibodies (R515n5, R514n5), and the cross-reactivity was 0.01%.

Time-resolved Fluorescent Immunoassay (TRFIA) platform

Roche-assigned fT3/tT3 clinical samples were detected using the T3 Sandwich antibody pair R513c8 (Capture) - R515n5 (Detection) on the Time-resolved Fluorescent Immunoassay (TRFIA) platform. A strong correlation (R² > 0.94) was confirmed between the detection results and Roche-assigned samples.

f/tT3 Sample Coincidence Rate

Thyroid function

3,3',5-Triiodo-L-thyronine (T3) is an important clinical indicator to evaluate thyroid function, which has important clinical significance in the diagnosis and efficacy detection of thyroid disease such as hyperthyroidism and hypothyroidism.
Research indicates that the level of free T3 (fT3) in serum can better reflect the clinical status than the total T3 level (the total T3 level changes with the concentration of carrier protein, while the fT3 level remains relatively constant). The increase of serum T3 concentration is mainly observed in hyperthyroidism. The combined detection of serum fT3 and serum free Thyroid hormones (fT4) can be used in the diagnosis, disease assessment and efficacy monitoring of hyperthyroidism and hypothyroidism.

Cardiovascular diseases

The concentration of serum free triiodothyronine (fT3) can reflect the activity of thyroid hormones. Thyroid function disorders can significantly increase the incidence rate and mortality of cardiovascular diseases. During long-term follow-ups, the incidence rate of cardiovascular diseases in the low fT3 level group was found significantly higher than that in the normal fT3 group, indicating that serum fT3 level is an important influencing factor for cardiovascular diseases, and low fT3 level indicates such risk may take place.

Moreover, the concentration of serum fT3 can directly affect the metabolism of blood lipids and blood glucose, and dyslipidemia can lead to the endothelial dysfunction. Research indicates that among familial hypercholesterolemia patients, the increase of cholesterol can lead to the disorder of endothelial function, which is one of the important mechanisms of early stage atherosclerosis.